CD55 antibody

Catalog No. ABIN94183

This Mouse Monoclonal antibody specifically detects CD55 in WB, FACS, IP and IHC (p). It exhibits reactivity toward Human and Non-Human Primate.It has been mentioned in 1 publication

Quick Overview for CD55 antibody (ABIN94183)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-118

Product Details anti-CD55 Antibody

Purpose: Anti-Hu CD55 Purified

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain extracellular glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: HPB-ALL human T cell line

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Recommended dilution: 10 μg/mL.
Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval, recommended dilution: 10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD55 antibody (ABIN94183)

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, (1999) (PubMed).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: CD55 Molecule (Cromer blood group),CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.,DAF, CROM, CHAPLE

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity