CD59 antibody (CD59) (FITC)

Details for Product anti-CD59 Antibody No. ABIN94201
Antigen
Reactivity
Human
456
27
26
10
8
7
3
2
2
1
1
Host
Mouse
329
156
15
8
Clonality (Clone)
Monoclonal ()
Conjugate
This CD59 antibody is conjugated to FITC
49
47
46
17
8
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
326
159
122
118
92
81
63
38
38
32
27
15
13
11
8
4
3
2
2
1
1
1
Options
Immunogen Thymocytes and T lymphocytes
Clone MEM-43
Isotype IgG2a
Specificity The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues. This antibody does not compete with MEM-43/5.
HLDA IV, WS Code NL 705
HLDA V, WS Code AS S013
HLDA V, WS Code BP BP345
HLDA V, WS Code T T-103
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Pathways Complement System
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD59 (CD59) antibody (FITC) (ABIN94201) Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.
Product cited in: Omidvar, Wang, Brennan, Longhi, Smith, Morgan: "Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2915-23, 2006 (PubMed).

Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Stefanová, Horejsí, Ansotegui, Knapp, Stockinger: "GPI-anchored cell-surface molecules complexed to protein tyrosine kinases." in: Science (New York, N.Y.), Vol. 254, Issue 5034, pp. 1016-9, 1991 (PubMed).

Forsberg, Bazil, Stefanová, Schröder: "Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11." in: Immunogenetics, Vol. 30, Issue 3, pp. 188-93, 1989 (PubMed).

Stefanová, Hilgert, Kristofová, Brown, Low, Horejsí: "Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol." in: Molecular immunology, Vol. 26, Issue 2, pp. 153-61, 1989 (PubMed).