CD59 antibody

Catalog No. ABIN94203

Cited in 3+ publications. The Mouse Monoclonal anti-CD59 antibody (Clone MEM-43-5) (ABIN94203) specifically detects CD59 in WB, FACS, IP and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for CD59 antibody (ABIN94203)

Target: CD59

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-43-5

Product Details anti-CD59 Antibody

Purpose: Anti-Hu CD59 Purified

Cross-Reactivity (Details): Mouse, Human

Characteristics: The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells, it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Thymocytes and T lymphocytes

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 0.5-4 μg/mL, positive control: blood.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: mouse spleen, non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD59 antibody (ABIN94203)

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Bodian, Davis, Morgan, Rushmere: "Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 507-16, (1997) (PubMed).

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

Gene ID: 966

UniProt: P13987

Pathways: Complement System