CD59 antibody (CD59)

Details for Product anti-CD59 Antibody No. ABIN94204
Antigen
Reactivity
Human, Pig (Porcine)
459
27
26
10
8
6
3
2
2
1
1
Host
Mouse
332
156
15
8
Clonality (Clone)
Monoclonal ()
Conjugate
This CD59 antibody is un-conjugated
50
47
47
17
8
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
329
160
122
119
93
83
65
38
38
32
28
15
13
11
8
4
3
2
2
1
1
1
Options
Immunogen Human peripheral blood lymphocytes
Clone MEM-129
Isotype IgM
Specificity The antibody MEM-129 reacts with CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues.
Cross-Reactivity (Details) Human, Porcine
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Pathways Complement System
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free HEPES buffered saline (HBS), approx. pH 7.0, 0.2 um filter sterilized.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997 (PubMed).

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, 1995 (PubMed).