Transferrin Receptor antibody

Catalog No. ABIN94226

The Mouse Monoclonal anti-Transferrin Receptor antibody has been validated for FACS, WB and IP. It is suitable to detect Transferrin Receptor in samples from Human. There is 1 publication available.

Quick Overview for Transferrin Receptor antibody (ABIN94226)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-189

Product Details anti-Transferrin Receptor Antibody

Purpose: Anti-Hu CD71 Purified

Specificity: The antibody MEM-189 reacts with an extracellular epitope on CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: KG1 human acute myelogenous leukaemia cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Transferrin Receptor antibody (ABIN94226)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71

Background: Transferrin receptor,CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.,TfR1, TfR, TR, Trfr, T9, p90, TFRC

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis