Transferrin Receptor (p90, CD71) (TFRC) antibody

Details for Product No. ABIN94226
Antigen
Reactivity
Human
575
166
47
11
6
6
5
5
5
4
3
2
1
1
Host
Mouse
450
156
108
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
73
63
62
30
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
482
280
187
176
141
109
57
45
42
26
12
8
8
6
2
2
2
1
1
1
1
Options
Immunogen KG1 human acute myelogenous leukaemia cell line
Clone MEM-189
Isotype IgG1
Specificity The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes.
The antibody MEM-189 does not block binding of transferrin to the receptor.
HLDA VI, WS Code NL N-L025
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD71 (TFRC Antibody Abstract)
Background CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.
Pathways Transition Metal Ion Homeostasis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, 2015 (PubMed).