CD99 antibody

Catalog No. ABIN94259

The Mouse Monoclonal anti-CD99 antibody has been validated for FACS, WB, IHC (p) and IP. It is suitable to detect CD99 in samples from Human. There is 1 publication available.

Quick Overview for CD99 antibody (ABIN94259)

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: MEM-131

Product Details anti-CD99 Antibody

Purpose: Anti-Hu CD99R Purified

Specificity: The antibody MEM-131 reacts with CD99R, an extracellular epitope restricted to a subset of CD99 Molecule expressed on myeloid cells, NK cells and T lymphocytes.

Cross-Reactivity (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: HPB-ALL human peripheral blood leukemia T-cell line

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymic lymphocytes.
Flow cytometry: Recommended dilution: 1 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD99 antibody (ABIN94259)

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, (1995) (PubMed).

Target Details for CD99

Target: CD99

Alternative Name: CD99R

Background: CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.,MIC2, HBA71, blood group Xg