CD99 antibody (CD99) (FITC)

Details for Product anti-CD99 Antibody No. ABIN94260
Antigen
Reactivity
Human
323
47
23
6
2
Host
Mouse
229
108
8
Clonality (Clone)
Monoclonal ()
Conjugate
This CD99 antibody is conjugated to FITC
25
22
12
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
202
118
118
112
75
32
28
22
16
13
8
4
4
2
1
Options
Immunogen HPB-ALL human peripheral blood leukemia T-cell line
Clone MEM-131
Isotype IgM
Specificity The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 Molecule expressed on myeloid cells, NK cells and T lymphocytes.
HLDA V, WS Code AS S020
HLDA V, WS Code T T-E2.02
HLDA V, WS Code T T-017
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD99R (CD99 Antibody Abstract)
Background CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD99 (CD99) antibody (FITC) (ABIN94260) Surface staining of human peripheral blood with anti-CD99R (MEM-131) FITC.
Product cited in: Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002 (PubMed).

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, 1995 (PubMed).