Keratin 7/17 antibody

Catalog No. ABIN94296

Cited in 1 publication. The Mouse Monoclonal anti-Keratin 7/17 antibody (Clone C-46) (ABIN94296) specifically detects Keratin 7/17 in WB, ICC, IHC (p) and IP. The antibody is reactive with Human, Cow and Pig samples.

Quick Overview for Keratin 7/17 antibody (ABIN94296)

Target: Keratin 7/17 (KRT7/17)

Reactivity: Human, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 7/17 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: C-46

Product Details anti-Keratin 7/17 Antibody

Purpose: Anti-Cytokeratin 7/17 Purified

No Cross-Reactivity: Mouse, Rabbit, Rat

Cross-Reactivity (Details): Porcine, Human, Bovine

Characteristics: The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, pretreatment: 0,1 % pepsin, 30 min, 37 °C, incubation 4 °C overnight, chromogen DAB, positive tissue: pancreas.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: HeLa cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Keratin 7/17 antibody (ABIN94296)

Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).

Target Details for Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Alternative Name: Cytokeratin 7/17

Background: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7

Gene ID: 35