HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) antibody

Details for Product No. ABIN94354
Antigen
Reactivity
Human, Non-Human Primate
131
8
7
6
6
2
1
1
1
Host
Mouse
66
66
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
12
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
71
41
32
24
24
20
13
2
2
1
Options
Immunogen Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Clone MEM-E-06
Isotype IgG1
Specificity The antibody MEM-E/06 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.
The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Cross-Reactivity (Details) Human, Non-Human Primates
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-E (HLAE Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) antibody (ABIN94354) Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (cl...
Product cited in: Das, Long: "Lytic granule polarization, rather than degranulation, is the preferred target of inhibitory receptors in NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 8, pp. 4698-704, 2010 (PubMed).

Dambaeva, Bondarenko, Grendell, Kravitz, Durning, Golos: "Non-classical MHC-E (Mamu-E) expression in the rhesus monkey placenta." in: Placenta, Vol. 29, Issue 1, pp. 58-70, 2008 (PubMed).

Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Palmisano, Contardi, Morabito, Gargaglione, Ferrara, Pistillo: "HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines." in: Human immunology, Vol. 66, Issue 1, pp. 1-12, 2004 (PubMed).

Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).