GAPDHS antibody

Catalog No. ABIN94395

The Mouse Monoclonal anti-GAPDHS antibody (Clone Hs-8) (ABIN94395) specifically detects GAPDHS in WB, FACS and ICC. The antibody is reactive with Human and Pig samples.

Quick Overview for GAPDHS antibody (ABIN94395)

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDHS antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: Hs-8

Product Details anti-GAPDHS Antibody

Purpose: Anti-GAPDHS Purified

Cross-Reactivity (Details): Human, Porcine

Characteristics: The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Isotype: IgM

Application Details

Application Notes: Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential.
Flow cytometry: Recommended dilution: 3-12 μg/mL. Intraacrosomal staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Alternative Name: GAPDHS

Background: Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35

Gene ID: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process