LIME antibody (AA 281-296)

Catalog No. ABIN94426

This Mouse Monoclonal antibody specifically detects LIME in WB, IHC (p) and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for LIME antibody (AA 281-296) (ABIN94426)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIME antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: LIME-10

Product Details anti-LIME Antibody

Binding Specificity: AA 281-296

Purpose: Anti-LIME1 Purified

Specificity: The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Western blotting: Reducing conditions, recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-LIME antibody (ABIN94426)

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1

Background: Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1

Gene ID: 54923

UniProt: Q9H400