CDK1 antibody

Catalog No. ABIN94446

This anti-CDK1 antibody is a Mouse Monoclonal antibody detecting CDK1 in WB, IP, IHC (p) and ICC. Suitable for Human, Cow and Non-Human Primate. This Primary Antibody has been cited in 1 publication.

Quick Overview for CDK1 antibody (ABIN94446)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Cow, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: POH-1

Product Details anti-CDK1 Antibody

Purpose: Anti-CDK1 Purified

Specificity: The antibody POH-1 specifically detects Cdk1 (p34Cdc2, an intracellular antigen). Staining of Cdk1 reflects the proliferating potential of respective tissue.

No Cross-Reactivity: Drosophila melanogaster, Mouse, Rat, Xenopus laevis

Cross-Reactivity (Details): Human, Non-Human Primates, Bovine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed full-length human Cdk1 protein

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymus.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CDK1 antibody (ABIN94446)

Lukás, Draetta, Bartek: "Distinct forms of human CDC2 identified by novel monoclonal antibodies." in: European journal of biochemistry / FEBS, Vol. 207, Issue 1, pp. 169-76, (1992) (PubMed).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1

Background: Cyclin dependent kinase 1,Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.,p34Cdc2, p34 protein kinase, CDK1, CDC2, CDC28A

Gene ID: 983

UniProt: B7Z3D6

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA