STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (AA 8-23)

Details for Product anti-STAT1 Antibody No. ABIN94469
Antigen
Epitope
AA 8-23
96
66
49
17
17
15
8
8
8
8
7
7
7
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
535
291
265
32
30
26
22
21
15
12
10
6
6
5
2
1
1
1
1
1
Host
Mouse
455
102
2
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This STAT1 antibody is un-conjugated
18
14
13
11
11
11
11
11
11
6
6
5
5
5
5
5
4
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
430
190
164
106
95
65
43
23
20
9
7
5
5
1
1
Options
Immunogen STAT1 peptide sequence 8-23 (QLDSKFLEQVHQLYD) conjugated to KLH.
Clone SM2
Isotype IgG1
Specificity The antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Cross-Reactivity (Details) Other not tested, Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.