ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (C-Term)

Details for Product anti-ZAP70 Antibody No. ABIN94502
Antigen
Epitope
C-Term
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54
50
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Reactivity
Human
566
234
169
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9
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1
Host
Rabbit
356
198
9
3
2
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
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26
14
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Application
Western Blotting (WB)
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14
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Options
Immunogen Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
Specificity The polyclonal antibody recognizes C-terminal part of human ZAP-70 protein tyrosine kinase.
ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Cross-Reactivity (Details) Human
Purification Purified from rabbit serum by affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Pathways TCR Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.