Amylase, alpha antibody

Catalog No. ABIN95003

Supplier Product No.: 100-4145

The Rabbit Polyclonal anti-Amylase, alpha antibody has been validated for WB, ELISA and IP. It is suitable to detect Amylase, alpha in samples from Bacillus amyloliquefaciens. There is 1 publication available.

Quick Overview for Amylase, alpha antibody (ABIN95003)

Target: Amylase, alpha (AMY)

Reactivity: Bacillus amyloliquefaciens

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylase, alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Amylase, alpha Antibody

Supplier Product No.: 100-4145

Supplier: Rockland

Purpose: Alpha Amylase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified a-Amylase [Bacillus amyloliquefaciens].

Characteristics: Synonyms: rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Purification: Anti-Alpha Amylase (Bacillus amyloliquefaciens) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: a-Amylase [Bacillus amyloliquefaciens]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Alpha Amylase is useful in ELISA, WB, and IP. User Optimization is suggested.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-Amylase, alpha antibody (ABIN95003)

van der Maarel, van der Veen, Uitdehaag, Leemhuis, Dijkhuizen: "Properties and applications of starch-converting enzymes of the alpha-amylase family." in: Journal of biotechnology, Vol. 94, Issue 2, pp. 137-55, (2002) (PubMed).

Target Details for Amylase, alpha

Target: Amylase, alpha (AMY)

Alternative Name: Alpha-amylase

Background: Background: Alpha Amylase is an enzyme that begins the digestion of starches. Specifically, Alpha Amylase cleaves the alpha bonds in large polysaccharides. Alpha Amylase is found in both the pancreas and saliva in humans, with salivary amylase beginning the digestion of starches and pancreatic amylase finishing the digestion. The large amount of conserved amino acid sequences and prevalence of alpha amylase enzymes has allowed this class of enzymes to be beneficial to industrial breakdown of starches into glucose and high-fructose corn syrup. Alpha amylase derived from bacillus amyloliquefaciens is also useful in the production of various detergents relying on the breakdown of starches. Anti-Alpha Amylase (Bacillus amyloliquefaciens) Antibody is ideal for investigators in Enzymology, Molecular Biology, and Microbiology research.

Gene ID: 7849308

NCBI Accession: WP_013352208

UniProt: P00692