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Amylase, alpha antibody (HRP)

AMY Reactivity: Bacillus amyloliquefaciens WB, ELISA, IP Host: Rabbit Polyclonal HRP
Catalog No. ABIN5596904
  • Target See all Amylase, alpha (AMY) Antibodies
    Amylase, alpha (AMY)
    Reactivity
    • 9
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Bacillus amyloliquefaciens
    Host
    • 16
    • 2
    Rabbit
    Clonality
    • 17
    • 1
    Polyclonal
    Conjugate
    • 9
    • 5
    • 1
    • 1
    • 1
    • 1
    This Amylase, alpha antibody is conjugated to HRP
    Application
    • 11
    • 9
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against a-Amylase from other tissues and species is unknown.
    Purification
    Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].
    Immunogen

    Immunogen: Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens a-Amylase protein.

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product AMY Primary Antibody
  • Application Notes

    Application Note: Alpha Amylase antibody has been assayed against 1.0 μg of Alpha Amylase [Bacillus amyloliquefaciens] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Amylase, alpha (AMY)
    Alternative Name
    Alpha Amylase (AMY Products)
    Synonyms
    LOC663954 antibody, LOC100136478 antibody, alpha amylase antibody, alpha-amylase antibody, LOC663954 antibody, HQ_RS05305 antibody, LOC100136478 antibody
    Background

    Synonyms: Alpha-amylase, EC=3.2.1.1, 1,4-alpha-D-glucan glucanohydrolase

    Background: Anti-Alpha Amylase Antibody detects Alpha Amylase. α-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Anti-Alpha Amylase Antibody is ideal for investigators involved in Cell Signaling.

    Gene Name: Alpha-amylase

    Gene ID
    7849308
    UniProt
    P00692
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