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beta Amyloid antibody (Amyloid beta) N-Term Primary Antibody

Abeta Reactivity: Human, Mouse IHC, ELISA, WB Host: Rabbit Polyclonal unconjugated
Pubmed (1 reference) Independent Validation (2 validations)
Catalog No. ABIN95037
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  • Target See all beta Amyloid (Abeta) Antibodies
    beta Amyloid (Abeta)
    Binding Specificity
    • 23
    • 21
    • 15
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 100
    • 29
    • 22
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 51
    • 47
    • 4
    • 2
    Rabbit
    Clonality
    • 51
    • 47
    • 1
    Polyclonal
    Conjugate
    • 88
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This beta Amyloid antibody is un-conjugated
    Application
    • 71
    • 65
    • 50
    • 27
    • 23
    • 21
    • 11
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Specificity
    This affinity-purified antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid. Cross reactivity with beta amyloid from other species is likely but has not been determined.
    Characteristics
    Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
    Purification
    affinity purified
    Sterility
    Sterile filtered
    Immunogen
    This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
    Immunogen Type: Peptide
    Isotype
    IgG
  • Application Notes
    Affinity purified anti-beta amyloid detects beta amyloid in ELISA, IHC and IF. A 40-50 kD band consistent with a higher MW precursor is detected in western blot using whole tissue extracts from mouse brain. In general, we recommend the use of 4% PFA for paraffin embedded tissues and 10% formalin for frozen tissue for fixation.
    Comment

    Gene Name: APP

    Restrictions
    For Research Use only
  • Validation #104082 (Unfolding Profile)
    'Independent Validation' Badge
    by
    NanoTemper Technologies
    No.
    #104082
    Date
    07/23/2019
    Antigen
    ABETA
    Lot Number
    Method validated
    Unfolding Profile
    Positive Control
    ABIN1043866
    Negative Control
    Notes

    Passed. ABIN1043866 showed Ti at 83.8°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    Secondary Antibody
    Full Protocol
    • Load the undiluted sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Experimental Notes

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Validation #104424 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104424
    Date
    05/12/2022
    Antigen
    beta Amyloid
    Lot Number
    42581
    Method validated
    Immunohistochemistry
    Positive Control

    Adult (24 months) mouse brain

    3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

    Negative Control

    We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

    Notes

    The beta Amyloid antibody (Amyloid beta) (N-Term) ABIN95037 works in IHC-P, especially at higher concentrations (1:20 and 1:50), and without the use of any antigen retrieval treatment.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN95037
    Secondary Antibody
    goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
    Full Protocol
    • Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
    • Wash, dehydrate, and embed samples in paraffin wax.
    • Wash several times with 0.01 M PBS.
    • Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
    • After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
    • Incubate sections with primary anti-beta amyloid (N-Term) (antibodies Online, ABIN95037, lot 42581) diluted 1:20, 1:50, 1:100, and 1:200 in 0.1 M PBS-BSA-PLL ON at RT.
    • Wash sections 3x 5 min with 0.01 M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
    • Wash sections 3x 5 min with 0.01M PBS.
    • Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    • Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
    Experimental Notes

    Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min (95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.

  • Format
    Liquid
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Expiry Date
    12 months
  • Frenkel, Balass, Solomon: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." in: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, (1998) (PubMed).

  • Target
    beta Amyloid (Abeta)
    Alternative Name
    Beta Amyloid (Abeta Products)
    Synonyms
    AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, aaa, abeta, abpp, ad1, appi, ctfgamma, cvap, pn2, Abeta, Abpp, Adap, Ag, Cvap, E030013M08Rik, betaApp, amyloid beta precursor protein, amyloid beta (A4) precursor protein, APP, app, App
    Background
    Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
    Synonyms: Beta amyloid, A-beta
    Gene ID
    351
    NCBI Accession
    NP_000475
    UniProt
    P05067
    Pathways
    Inflammasome
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