CD226 antibody (FITC)

Catalog No. ABIN951229

Product Details anti-CD226 Antibody (FITC)

Target: CD226

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD226 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Recent experiments show that this antibody (clone 15F.10E5) only binds to differentiated Th1 cells but not Th2 or Th0 cells. It also suppressed both antigen-specific T cell expansion and an autoimmune disease (EAE) mediated by effector Th1 cells.

Immunogen: AE7 cells Donor: Lewis rat Fusion Partner: SP 2/0 myeloma

Clone: 15F-10E5

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD226

Target: CD226

Alternative Name: CD226 / DNAM1 (CD226 Products)

Background: CD226 (also called DNAM-1) is constituitively expressed on naive CD8+ T cells, as well as subsets of naive CD11b+ macrophages and NK cells. It is also found on a lower percentage (~40 % ) of unactivated CD4+ T cells. CD226 is functional upon T cell activation, and CD226 ligands (CD112 and CD155) are the same in the mouse as for human (Tage-4 is the mouse homologue of CD155). The gene for mouse DNAM-1 was identified on chromosome 18, and the predicted amino acid sequence shows a 53 % homology with human DNAM-1.Synonyms: DNAM-1, DNAX accessory molecule 1

Gene ID: 225825

NCBI Accession: NP_848802

UniProt: Q8K4F0

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints