CD226 antibody (pSer329)

Catalog No. ABIN1531541

The Rabbit Polyclonal anti-CD226 antibody is suitable to detect CD226 in samples from Human, Mouse and Monkey. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CD226 antibody (pSer329) (ABIN1531541)

Target: CD226

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD226 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD226 Antibody

Binding Specificity: AA 287-336, pSer329

Purpose: DNAM-1 (phospho Ser329) Antibody

Specificity: Phospho-DNAM-1 (S329) Polyclonal Antibody detects endogenous levels of DNAM-1 protein only when phosphorylated at S329.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1 around the phosphorylation site of Ser329. AA range:287-336

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CD226

Target: CD226

Alternative Name: CD226/DNAM-1

Background:

Synonyms: CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD antigen CD226

Background: This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],

Gene Name: CD226

Protein Name: CD226 antigen

Molecular Weight: 38 kDa

Gene ID: 10666

OMIM: 605397

UniProt: Q15762

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints