CD25 antibody (FITC)

Catalog No. ABIN951248

The Rat Monoclonal anti-CD25 antibody has been validated for FACS. It is suitable to detect CD25 in samples from Mouse.

Quick Overview for CD25 antibody (FITC) (ABIN951248)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: PC61-5-3

Product Details anti-CD25 Antibody (FITC)

Specificity: This monoclonal antibody reacts with the low affinity alpha chain of the Interleukin-2 receptor antigen present on activated T and B cells in mice. AM26037FC inhibits IL-2 binding and IL-2 dependent proliferation.

Purification: Affinity chromatography on Protein G

Immunogen: B6.1 CTL Cell line Donor: OFA rat spleen Fusion Partner: mouse myeloma line P3X63Ag8.653

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation