DENND1B antibody (N-Term)

Catalog No. ABIN951885

This anti-DENND1B antibody is a Rabbit Polyclonal antibody detecting DENND1B in WB, FACS, IHC (p) and EIA. Suitable for Human.

Quick Overview for DENND1B antibody (N-Term) (ABIN951885)

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DENND1B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DENND1B Antibody

Binding Specificity: AA 46-76, N-Term

Specificity: This antibody recognizes Human DENND1B (N-term).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of Human DENND1B. Genename: DENND1B

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Alternative Name: DENND1B

Background: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2, see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).Synonyms: C1orf218, Connecdenn 2, DENN domain-containing protein 1B, FAM31B

Molecular Weight: 48260 Da

Gene ID: 163486

NCBI Accession: NP_001182144