E2F2 antibody (Middle Region)

Catalog No. ABIN952013

The Rabbit Polyclonal anti-E2F2 antibody is suitable to detect E2F2 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for E2F2 antibody (Middle Region) (ABIN952013)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-E2F2 Antibody

Binding Specificity: AA 265-297, Middle Region

Specificity: This antibody recognizes Human and Mouse E2F2 (Center).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 265-297 amino acids from the Central region of Human E2F2

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F2

Background: E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1. [provided by RefSeq].Synonyms: E2F transcription factor 2, E2F-2, Transcription factor E2F2

Molecular Weight: 47506 Da

Gene ID: 1870

NCBI Accession: NP_004082

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication