ATG12 antibody (Autophagy Related 12)

Details for Product anti-ATG12 Antibody No. ABIN95224
Antigen
Reactivity
Yeast (Saccharomyces cerevisiae)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This ATG12 antibody is un-conjugated
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Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Options
Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name APG12 (ATG12 Antibody Abstract)
Background Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.
Synonyms: APG12-like antibody, APG12L antibody, ATG12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog antibody, Autophagy 12 antibody, Autophagy-related protein 12 antibody, HAPG12 antibody
Gene ID 852518, 6319694
UniProt P38316
Pathways Autophagy
Application Notes This purified polyclonal antibody reacts yeast APG12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates).  Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 5.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C