Sca-1/Ly-6A/E antibody (PE)

Catalog No. ABIN953252

This Rat Monoclonal antibody specifically detects Sca-1/Ly-6A/E in FACS. It exhibits reactivity toward Mouse.

Quick Overview for Sca-1/Ly-6A/E antibody (PE) (ABIN953252)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: CT-6A-6E

Product Details anti-Sca-1/Ly-6A/E Antibody (PE)

Specificity: This monoclonal antibody recognizes Sca-1 (Ly-6A.2/6E.1), a cell surface antigen used in the identification of hematopoietic stem cells.

Purification: Affinity chromatography on Protein G

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS containing 0.02 % sodium azide (NaN3) as preservative A highly purified grade of BSA has been added as a stablizing protein to bring the final protein concentration to 4-5 mg/mL after conjugation.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly6A/E / SCA1

Background: Ly6A/E is a member of the Ly-6 antigen family. The Thy-1lo, Lin- (lineage-negative, not expressing B220, Gr-1, Mac-1, CD4 or CD8), Sca-1+ population of bone marrow cells are highly purified, perhaps homogenous, pluripotent stem cells. This antigen is also present on various other tissues. Specific staining of the parenchymal cells can be demonstrated in thymus, spleen and kidney where as only vasculature reacts with anti-Sca-1 in brain, heart and liver (and possibly in lung). Also, Sca-1 is a T cell activation antigen, as surface expression of the antigen increases upon Con A activation of T lymphocytes. Sca-1 appears to have a molecular mass of 8 kDa under non-reducing conditions and 18 kDa under reducing conditions, indicating the presence of intra-chain disulfide bonds.Synonyms: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP

Gene ID: 110454

NCBI Accession: NP_034868

UniProt: P05533

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation