JNK antibody (AA 162-199)

Catalog No. ABIN953314

This Rabbit Polyclonal antibody specifically detects JNK in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for JNK antibody (AA 162-199) (ABIN953314)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-JNK Antibody

Binding Specificity: AA 162-199

Specificity: This antibody recognizes Human and Mouse MAPK8 / JNK1 (Thr183/Tyr185).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 162-199 amino acids from human MAPK8 / JNK1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8 / JNK1

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: JNK-1, JNK-46, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1

Molecular Weight: 48296 Da

Gene ID: 5599

NCBI Accession: NP_002741

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins