MRPS15 antibody (Middle Region)

Catalog No. ABIN953517

The Rabbit Polyclonal anti-MRPS15 antibody (ABIN953517) specifically detects MRPS15 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for MRPS15 antibody (Middle Region) (ABIN953517)

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS15 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MRPS15 Antibody

Binding Specificity: AA 123-153, Middle Region

Specificity: This antibody recognizes human MRPS15.

Purification: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 123-153aa) of human MRPS15.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MRPS15

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Alternative Name: MRPS15

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The MRPS15 gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.Synonyms: 28S ribosomal protein S15, DC37, RPMS15, S15mt, mitochondrial

Molecular Weight: 29842 Da

Gene ID: 64960

NCBI Accession: NP_112570