Nucleolin antibody (Middle Region)

Catalog No. ABIN953773

This Rabbit Polyclonal antibody specifically detects Nucleolin in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for Nucleolin antibody (Middle Region) (ABIN953773)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Nucleolin Antibody

Binding Specificity: AA 435-463, Middle Region

Specificity: This antibody recognizes Human Nucleolin (Center).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of Human Nucleolin. Genename: NCL

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20Synonyms: NCL

Molecular Weight: 76614 Da

Gene ID: 4691

NCBI Accession: NP_005372

Pathways: Ribonucleoprotein Complex Subunit Organization