Nucleolin antibody (NCL) (AA 435-463)

Details for Product anti-NCL Antibody No. ABIN953773
Antigen
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Epitope
AA 435-463, Middle Region
14
14
11
10
9
8
8
7
7
7
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
239
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
131
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
219
140
133
98
72
60
42
25
21
17
13
9
9
2
1
1
1
Options
Immunogen KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of Human Nucleolin. Genename: NCL
Isotype Ig Fraction
Specificity This antibody recognizes Human Nucleolin (Center).
Purification Protein A column, followed by peptide affinity purification
Plasmids, Primers & others Plasmids, Primers & others Nucleolin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20Synonyms: NCL
Molecular Weight 76614 Da
Gene ID 4691
NCBI Accession NP_005372
Pathways Ribonucleoprotein Complex Subunit Organization
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Image no. 1 for anti-Nucleolin (NCL) (AA 435-463), (Middle Region) antibody (ABIN953773) Confocal immunofluorescent analysis of Nucleolin Antibody (Center) Cat.-No AP52825PU-...
Image no. 2 for anti-Nucleolin (NCL) (AA 435-463), (Middle Region) antibody (ABIN953773) Formalin fixed, paraffin embedded human breast tissue stained with Nucleolin Antibody...
Image no. 3 for anti-Nucleolin (NCL) (AA 435-463), (Middle Region) antibody (ABIN953773) Western blot analysis of NCL (arrow) using Nucleolin Antibody (Center) Cat.-No AP5282...
Image no. 4 for anti-Nucleolin (NCL) (AA 435-463), (Middle Region) antibody (ABIN953773) Western blot analysis of Nucleolin Antibody (Center) in HepG2 cell line lysates (35ug...
Background publications Tulchin, Chambon, Juan, Dikman, Strauchen, Ornstein, Billack, Woods, Monteiro: "BRCA1 protein and nucleolin colocalize in breast carcinoma tissue and cancer cell lines." in: The American journal of pathology, Vol. 176, Issue 3, pp. 1203-14, 2010 (PubMed).

Strang, Boulant, Coen: "Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication." in: Journal of virology, Vol. 84, Issue 4, pp. 1771-84, 2010 (PubMed).

Ishimaru, Zuraw, Ramalingam, Sengupta, Bandyopadhyay, Reuben, Fernandes, Spicer: "Mechanism of regulation of bcl-2 mRNA by nucleolin and A+U-rich element-binding factor 1 (AUF1)." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27182-91, 2010 (PubMed).

Bertrand, Leiva-Torres, Hyjazie, Pearson: "Conserved residues in the UL24 protein of herpes simplex virus 1 are important for dispersal of the nucleolar protein nucleolin." in: Journal of virology, Vol. 84, Issue 1, pp. 109-18, 2009 (PubMed).

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