Vaccinia Virus antibody (Biotin)

Catalog No. ABIN955498

This Rabbit Polyclonal antibody specifically detects Vaccinia Virus in EIA and IHC (p). It exhibits reactivity toward Vaccinia Virus (VACV).

Quick Overview for Vaccinia Virus antibody (Biotin) (ABIN955498)

Target: Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Reactivity: Vaccinia Virus (VACV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vaccinia Virus antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vaccinia Virus Antibody (Biotin)

Purification: Protein A Chromatography

Immunogen: Lister Strain (mixture of virions and ICPs)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS pH 7.2 containing 0.09 % Sodium Azide as preservative. Contains no stabilizing proteins.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C only.

Target Details for Vaccinia Virus

Target: Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Alternative Name: Vaccinia Virus

Target Type: Virus

Background: Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.