Vimentin antibody

Catalog No. ABIN955516

This Chicken Polyclonal antibody specifically detects Vimentin in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Vimentin antibody (ABIN955516)

Target: Vimentin (VIM)

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vimentin Antibody

Specificity: Recognizes Vimentin.

Purification: Affinity Chromatography using a peptide column.

Immunogen: Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.3 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Human vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimenin is reexpressed by the tumor cells and serves as a general indicator of their undifferentiated state.Synonyms: VIM

Gene ID: 7431

NCBI Accession: NP_003371

Pathways: Caspase Cascade in Apoptosis