ABCB1 antibody

Catalog No. ABIN956743

This Mouse Monoclonal antibody specifically detects ABCB1 in IHC, IHC (p), FACS and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ABCB1 antibody (ABIN956743)

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LRP-56

Product Details anti-ABCB1 Antibody

Specificity: LRP-56 reacts with an internal epitope of the LRP-protein (P110), which is strongly over-expressed in various human non-P-glycoprotein MDR tumor cell lines.

Purification: Purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr, IHC-P

Usage: LRP-56 has potential value for detection of LRP-associated non-Pgp MDR in human tumor samples. Flow cytometry: at least 1:50. Immunocytochemistry: acetone fixed cell preparation at least 1:20-50. Immunohistochemistry: acetone-fixed frozen sections at least 1:20-50, formalin-fixed paraffin embedded tissues 1:20. In Flow cytometry fixate cells in 10 % (v/v) Lysing solution followed by primary antibody and anti-Mouse-FITC.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 1 % BSA, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: ABCB1 / MDR1 / P Glycoprotein

Background: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

Gene ID: 5243

UniProt: P08183