alpha Tubulin antibody (Subunit alpha)

Catalog No. ABIN960542

The Rat Monoclonal anti-alpha Tubulin antibody has been validated for WB, ELISA, IHC, IHC (p), IHC (fro) and RIA. It is suitable to detect alpha Tubulin in samples from Human, Saccharomyces cerevisiae, Mammalian, Avian, Drosophila melanogaster and Fungus.

Quick Overview for alpha Tubulin antibody (Subunit alpha) (ABIN960542)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Saccharomyces cerevisiae, Mammalian, Avian, Drosophila melanogaster, Fungus

Host: Rat

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Clone: YOL1-34

Product Details anti-alpha Tubulin Antibody

Binding Specificity: Subunit alpha

Brand: IHC-plus™

Specificity: Recognizes the alpha subunit of tubulin. This product is routinely tested in ELISA on Tubulin.

Purification: Affinity purified

Immunogen: Yeast tubulin.

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (10 μg/mL), IHC, IHC-Fr, IHC-P (10 μg/mL), RIA, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin

Pathways: Microtubule Dynamics