Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (N-Term)

Details for Product anti-CASP2 Antibody No. ABIN96091
Antigen
Epitope
N-Term
14
12
8
8
8
6
6
6
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
225
116
87
14
10
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
202
17
16
Clonality
Polyclonal
Conjugate
This Caspase 2 antibody is un-conjugated
11
7
5
5
5
5
5
5
3
2
2
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
174
87
71
38
22
16
15
14
11
4
4
4
4
2
Options
Immunogen A synthetic peptide corresponding to the of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name Caspase-2 (CASP2 Antibody Abstract)
Background Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure.  Disruption of apoptosis may contribute to cancer and other diseases.  Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse.  Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis.  The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits.  The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.  Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B.  Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7.  Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue.  Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2.  These forms show tissue specificity and function either as inducers or suppressors of apoptosis.  Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
Synonyms: Apoptosis related cysteine peptidase antibody, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Developmentally down regulated 2 antibody, ICH 1 antibody
Gene ID 64314
UniProt P55215
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway
Application Notes This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2.  A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2.  No reaction was observed against human caspase-2.  Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2.This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot.
Restrictions For Research Use only
Format Liquid
Concentration 0.44 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (N-Term) antibody (ABIN96091) Affinity Purified antibody to Caspase-2  was used at a 1:750 dilution to detect rat c...