Caspase 2 antibody (N-Term)

Catalog No. ABIN96091

Product Details anti-Caspase 2 Antibody

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Binding Specificity: N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: A synthetic peptide corresponding to the of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

Isotype: IgG

Application Details

Application Notes: This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2.  A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2.  No reaction was observed against human caspase-2.  Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2.This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.44 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Alternative Name: Caspase-2 (CASP2 Products)

Synonyms: xCaspase-2 antibody, Caspase-2 antibody, ICH-1 antibody, Nedd2 antibody, CASP-2 antibody, ICH1 antibody, NEDD-2 antibody, NEDD2 antibody, PPP1R57 antibody, ICH-1L/1S antibody, ICH1L1S antibody, caspase 2 antibody, caspase 2 L homeolog antibody, caspase-2 antibody, CASP2 antibody, casp2.L antibody, CpipJ_CPIJ008254 antibody, Casp2 antibody

Background: Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure.  Disruption of apoptosis may contribute to cancer and other diseases.  Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse.  Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis.  The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits.  The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.  Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B.  Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7.  Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue.  Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2.  These forms show tissue specificity and function either as inducers or suppressors of apoptosis.  Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
Synonyms: Apoptosis related cysteine peptidase antibody, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Developmentally down regulated 2 antibody, ICH 1 antibody

Gene ID: 64314

UniProt: P55215

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway