CRYAB antibody (pSer59)

Catalog No. ABIN961132

Product Details anti-CRYAB Antibody

Target: CRYAB (Crystallin, alpha B (CRYAB))

Binding Specificity: AA 54-64, pSer59

Reactivity: Human, Mouse, Rat, Cow, Pig, Sheep, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody detects an ~22kD protein, corresponding to the apparent molecular mass of phosphorylated alpha-B-crystallin on SDS-PAGE immunoblots, in samples from mouse, rat, bovine, canine, porcine and sheep. There is no reactivity with non-phosphorylated alpha B-crystallin. This antibody may cross react with beta crystallin.

Purification: Purified

Immunogen: Human synthetic peptide corresponding to amino acids 54-64 and containing phosphorylated serine 59. This peptide is conserved among mouse, rat and bovine.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP (12.5 μg/mL), WB

Usage: Western Blot (Colorimetric): 2 μg/mL. Immunoprecipitation: 12.5 μg/mL. Positive control: Alpha Crystallin Protein.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 3 % BSA, 0.1 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB / Alpha B Crystallin (CRYAB Products)

Synonyms: CMD1II antibody, CRYA2 antibody, CTPP2 antibody, CTRCT16 antibody, HSPB5 antibody, MFM2 antibody, Crya-2 antibody, Crya2 antibody, HspB5 antibody, AACRYA antibody, CRYAB antibody, cryab antibody, cryab2 antibody, wu:fe37f08 antibody, zgc:91937 antibody, crystallin alpha B antibody, crystallin, alpha B antibody, hypothetical protein antibody, crystallin, alpha B, a antibody, crystallin, alpha B, b antibody, CRYAB antibody, Cryab antibody, ZK1128.7 antibody, cryaba antibody, cryabb antibody

Background: Name/Gene ID: CRYAB

Synonyms: CRYAB, Alpha-crystallin B chain, CRYA2, Crystallin, alpha B, CTPP2, Heat shock protein beta-5, HSPB5, Alpha B Crystallin, Alpha(B)-crystallin, Rosenthal fiber component, Heat-shock 20 kD like-protein

Gene ID: 1410

UniProt: P02511