GST antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN964665

Supplier Product No.: 600-405-200

This anti-GST antibody is a Rabbit Polyclonal antibody detecting GST in WB, ELISA and IHC. Suitable for Schistosoma japonicum.

Quick Overview for GST antibody (Alkaline Phosphatase (AP)) (ABIN964665)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GST antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GST Antibody (Alkaline Phosphatase (AP))

Supplier Product No.: 600-405-200

Supplier: Rockland

Purpose: GST Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Characteristics: Synonyms: rabbit anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200-1:1,000

Application Note: Anti-Glutathione-S-Transferase Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500-1:2,500

ELISA Dilution: 1:2,000-1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST

Background: Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.