GDF15 antibody (C-Term)

Catalog No. ABIN964730

Supplier Product No.: 200-501-b07

This anti-GDF15 antibody is a Rat Monoclonal antibody detecting GDF15 in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for GDF15 antibody (C-Term) (ABIN964730)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GDF15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 6D12-H10-E4

Product Details anti-GDF15 Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-501-b07

Supplier: Rockland

Purpose: GDF15 Antibody

Cross-Reactivity (Details): This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues.

Characteristics: Synonyms: rat anti-NAG1 antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

Purification: This product was purified from concentrated tissue culture supernatant Protein G chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein.  Cross reactivity to MBP is negative.

Immunogen Type: Recombinant Protein

Isotype: IgG2a lambda

Application Details

Application Notes:

Application Note: This Protein G Anti-NAG1/GDF15 purified antibody has been tested by ELISA and western blot for mouse NAG-1 protein.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of native protein of approximately ~58 kDa using the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome