FIL1d antibody

Catalog No. ABIN964734

Supplier Product No.: 201-401-b77

This anti-FIL1d antibody is a Rabbit Polyclonal antibody detecting FIL1d in WB and ELISA. Suitable for Cow.

Quick Overview for FIL1d antibody (ABIN964734)

Target: FIL1d (Interleukin 1 delta (FIL1d))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FIL1d antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FIL1d Antibody

Supplier Product No.: 201-401-b77

Supplier: Rockland

Purpose: IL-1F5 Antibody

Cross-Reactivity (Details): This antibody is specific for bovine IL-1F5 protein.

Characteristics: Synonyms: rabbit anti-IL-1F5 antibody, rabbit anti-Interleukin 1 family, member 5 (Delta)

Purification: This product was Protein-A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified Interleukin-1F5 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kDa in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 8,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 µg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FIL1d

Target: FIL1d (Interleukin 1 delta (FIL1d))

Alternative Name: IL1F5

Background: Background: Interleukin-1F5 is a recently discovered cytokine which was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6) in humans. There is very little published regarding the bovine IL1F5 protein. In the human, this gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Expression of the gene appears to be restricted to various epithelial cell populations. Costelloe et al (2008) have identified SIGIRR [single immunoglobulin domain-containing IL1R-related protein] as a receptor for human IL1F5. Anti-IL-1F5 antibody is ideal for investigators involved in Signal Transduction and Immunology research.

Gene ID: 518514, 115494990

UniProt: Q0VC52