GDF15 antibody

Catalog No. ABIN964753

Supplier Product No.: 209-401-b88

The Rabbit Polyclonal anti-GDF15 antibody has been validated for WB and ELISA. It is suitable to detect GDF15 in samples from Human and Mouse.

Quick Overview for GDF15 antibody (ABIN964753)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GDF15 Antibody

Supplier Product No.: 209-401-b88

Supplier: Rockland

Purpose: GDF15 Antibody

Cross-Reactivity (Details): This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100 % and 98 % homology, respectively, and with macaque based on an 94 % homology, and with mouse and rat based on 70 % homology with the immunizing sequence.

Characteristics: Synonyms: rabbit anti-NAG-1 Antibody, NAG1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

Sterility: Sterile filtered

Immunogen:

Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein.  Cross reactivity to MBP was removed via cross-adsorption chromatography.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This Protein-A purified, MBP-cross-adsorbed NAG1/GDF15 antibody has been tested by ELISA and Western Blot of human and mouse NAG-1 protein.   For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG1/GDF15 C-terminal specific antibodies.  This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.  Expect bands in western blots of approximately 14 kDa in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.

Western Blot Dilution: 1:800 - 1:1500

ELISA Dilution: 1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.37 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

Gene ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome