CCL4 antibody

Catalog No. ABIN964800

Supplier Product No.: 214-401-b85

Product Details anti-CCL4 Antibody

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 214-401-b85

Supplier: Rockland

Purpose: MIP-1 beta Antibody

Cross-Reactivity (Details): This antibody is specific for swine CCL4 protein.

Characteristics: Synonyms: rabbit anti-MIP 1 beta Antibody, rabbit anti-MIP-1b Antibody, rabbit anti-CCL4 antibody, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, MIP-1ß

Purification: This product was Protein-A purified from monospecific antiserum by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1ß protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified MIP-1ß antibody has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C upon receipt. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4 (CCL4 Products)

Background: Background: In many species, both C-C chemokines macrophage inflammatory protein, or MIP-1 alpha (CCL3L1), and MIP-1 beta (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1 alpha selectively attracts CD8+ lymphocytes, while MIP-1 beta selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1 beta (CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells.

Gene ID: 396668

UniProt: Q711P4