ASK1 antibody

Catalog No. ABIN965600

Product Details anti-ASK1 Antibody

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: ASK1 (Ab-83) antibody detects endogenous levels of ASK1 protein around serine 83.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83 (G-S-SP-V-G).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ASK1 antibody (ABIN965600)

Yuan, Feldman, Sussman, Coppola, Nicosia, Cheng: "AKT2 inhibition of cisplatin-induced JNK/p38 and Bax activation by phosphorylation of ASK1: implication of AKT2 in chemoresistance." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23432-40, (2003) (PubMed).

Mabuchi, Ohmichi, Kimura, Nishio, Arimoto-Ishida, Yada-Hashimoto, Tasaka, Murata: "Estrogen inhibits paclitaxel-induced apoptosis via the phosphorylation of apoptosis signal-regulating kinase 1 in human ovarian cancer cell lines." in: Endocrinology, Vol. 145, Issue 1, pp. 49-58, (2003) (PubMed).

Kim, Khursigara, Sun, Franke, Chao: "Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1." in: Molecular and cellular biology, Vol. 21, Issue 3, pp. 893-901, (2001) (PubMed).

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1 (MAP3K5 Products)

Synonyms: ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response