beta Actin antibody (N-Term)

Catalog No. ABIN965658

Cited in 2+ publications. The Mouse Monoclonal anti-beta Actin antibody (Clone ac-15) (ABIN965658) specifically detects beta Actin in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for beta Actin antibody (N-Term) (ABIN965658)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Ac-15

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Immunogen: slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 2.2 % sodium acetate or neutral PBS. If 1 mL of PBS is used, the antibody concentration will be 100 µg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-beta Actin antibody (ABIN965658)

Ng, Gunning, Eddy, Ponte, Leavitt, Shows, Kedes: "Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes." in: Molecular and cellular biology, Vol. 5, Issue 10, pp. 2720-32, (1986) (PubMed).

Tardieu, Virot, Vandroux, Vergnoux, Doumeix, Delhoume, Chabanier, Blanc, Richard, Bensaid: "[A case of electrically and mechanically stunned myocardium]." in: Archives des maladies du coeur et des vaisseaux, Vol. 78, Issue 6, pp. 947-50, (1985) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin

Background: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Gene ID: 60, 11461, 81822

UniProt: P60710, P60711, P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction