CD4 antibody

Catalog No. ABIN965795

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS and IHC (fro). It is suitable to detect CD4 in samples from Human. There is 1 publication available.

Quick Overview for CD4 antibody (ABIN965795)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B486 (EDU-2)

Product Details anti-CD4 Antibody

Specificity: This antibody reacts with a 55kD single chain transmembrane glycoprotien, CD4, expressed on T helper lymphocytes, dendritic cells, microglial cells, and cortical thymus cells. Characteristic of CD4 is its four extracellular immunoglobulin-like domains which help it bind to HLA class II molecules when CD4+ T cells interact with antigenpresenting cells or target cells. Another interesting aspect of the CD4 receptor is the fact that it also serves as the primary receptor for the HIV virus. This antibody was clustered at the 2nd International Leucocyte Typing Workshop.

Purification: Concentrated.

Immunogen: Human leucocytes

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.(Frozen sections only)
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen sections only. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD4 antibody (ABIN965795)

Zosimova: "[Gas chromatographic detection of benzene, toluene and xylene in biological matter]." in: Sudebno-meditsinskaia ekspertiza, Vol. 29, Issue 3, pp. 37-8, (1986) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

Gene ID: 920

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events