CHEK2 antibody

Catalog No. ABIN965891

The Rabbit Polyclonal anti-CHEK2 antibody has been validated for IHC. It is suitable to detect CHEK2 in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for CHEK2 antibody (ABIN965891)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-CHEK2 Antibody

Specificity: Chk2 (Ab-68) antibody detects endogenous levels of total Chk2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 68.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CHEK2 antibody (ABIN965891)

Bhoumik, Takahashi, Breitweiser, Shiloh, Jones, Ronai: "ATM-dependent phosphorylation of ATF2 is required for the DNA damage response." in: Molecular cell, Vol. 18, Issue 5, pp. 577-87, (2005) (PubMed).

Gorgoulis, Vassiliou, Karakaidos, Zacharatos, Kotsinas, Liloglou, Venere, Ditullio, Kastrinakis, Levy, Kletsas, Yoneta, Herlyn, Kittas, Halazonetis: "Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions." in: Nature, Vol. 434, Issue 7035, pp. 907-13, (2005) (PubMed).

Jin, Kurosu, Yamaguchi, Arai, Miura: "Hematopoietic cytokines enhance Chk1-dependent G2/M checkpoint activation by etoposide through the Akt/GSK3 pathway to inhibit apoptosis." in: Oncogene, Vol. 24, Issue 12, pp. 1973-81, (2005) (PubMed).

Li, Stern: "Regulation of CHK2 by DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 12041-50, (2005) (PubMed).

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle