KRT8 antibody (AA 391-483)

Catalog No. ABIN965965

This Mouse Monoclonal antibody specifically detects KRT8 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for KRT8 antibody (AA 391-483) (ABIN965965)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: 8A5D12

Product Details anti-KRT8 Antibody

Binding Specificity: AA 391-483

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-KRT8 antibody (ABIN965965)

Wang, He, Tsao, Cheung, Wong, Chiu: "Cytokeratin 8 silencing in human nasopharyngeal carcinoma cells leads to cisplatin sensitization." in: Cancer letters, Vol. 265, Issue 2, pp. 188-96, (2008) (PubMed).

Walker, Harris, Holloway, McKenzie, Wells, Robinson, Morris: "Cytokeratin KRT8/18 expression differentiates distinct subtypes of grade 3 invasive ductal carcinoma of the breast." in: Cancer genetics and cytogenetics, Vol. 178, Issue 2, pp. 94-103, (2007) (PubMed).

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Synonyms: CK8, CYK8, K2C8, KRT8

Molecular Weight: 54kDa

Gene ID: 3856