KRT8 antibody

Catalog No. ABIN965966

The Mouse Monoclonal anti-KRT8 antibody has been validated for IHC (p) and IHC (fro). It is suitable to detect KRT8 in samples from Human.

Quick Overview for KRT8 antibody (ABIN965966)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B391

Product Details anti-KRT8 Antibody

Purification: Concentrated.

Immunogen: Cytoskeleton preparation from HeLa Cells

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50 -1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III. The optimal conditions should be determined by the individual laboratory. The Auto/Zyme predigestion or high temperature antigenic unmasking technique is recommended with this antibody when using paraffinembedded tissue sections.

Comment:

Cellular Localization: Cytoplasm.
Recommended Positive Control: Colon, Lung, or Breast Carcinomas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 35 mg/ml

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8 (52.2 kDa)

Background: This antibody reacts with Cytokeratin 8 (52 and 45 kD) human polypeptide. This cytokeratin coexists With Cytokeratin 18 and is found in all non-squamous epithelium but not in squamous epithelium. It Reacts with the adenocarcinomas of ovary, gastrointestinal tract, prostate, and thyroid.