EGF antibody (Epidermal Growth Factor)

Details for Product anti-EGF Antibody No. ABIN966031, Supplier: Log in to see
Antigen
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Alternatives
anti-Rat (Rattus) EGF antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
381
186
109
20
17
9
4
2
2
1
1
1
Host
Mouse
370
147
32
26
6
3
Clonality (Clone)
Monoclonal ()
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
417
281
158
101
54
33
30
30
27
27
23
16
14
7
7
5
4
3
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Clone 4E11
Isotype IgG1
Purification Ascitic fluid
Plasmids, Primers & others Plasmids, Primers & others EGF products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Synonyms: URG, HOMG4, EGF
Molecular Weight 134kDa
Gene ID 1950
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Application Notes Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003 (PubMed).

Background publications Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002 (PubMed).

Gual, Giordano, Williams, Rocchi, Van Obberghen, Comoglio: "Sustained recruitment of phospholipase C-gamma to Gab1 is required for HGF-induced branching tubulogenesis." in: Oncogene, Vol. 19, Issue 12, pp. 1509-18, 2000 (PubMed).

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993 (PubMed).

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