ERK2 antibody

Catalog No. ABIN966090

Cited in 2+ publications. The Monoclonal anti-ERK2 antibody (ABIN966090) specifically detects ERK2 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for ERK2 antibody (ABIN966090)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ERK2 Antibody

Specificity: Ni-NTA purified full length recombinant ERK2 expressed in E. Coli strain BL21 (DE3)

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-ERK2 antibody (ABIN966090)

Whitehurst, Robinson, Moore, Cobb: "The death effector domain protein PEA-15 prevents nuclear entry of ERK2 by inhibiting required interactions." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12840-7, (2004) (PubMed).

Begum, Asghar, N, Khan, Khan: "High dose rate intraluminal brachytherapy in combination with external beam radiotherapy for palliative treatment of cancer rectum." in: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP, Vol. 13, Issue 11, pp. 633-6, (2003) (PubMed).

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: ERK2

Background: ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Gene ID: 5594

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins