ERK2 antibody (C-Term)

Catalog No. ABIN966091

Product Details anti-ERK2 Antibody

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 1 (MAPK1) detection. Tested with WB in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human ERK2, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-ERK2 antibody (ABIN966091)

Oliveira, Rigon, Leal, Rossi: "The activation of ERK1/2 and p38 mitogen-activated protein kinases is dynamically regulated in the developing rat visual system." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 26, Issue 3-4, pp. 355-62, (2008) (PubMed).

Forcet, Stein, Pays, Corset, Llambi, Tessier-Lavigne, Mehlen: "Netrin-1-mediated axon outgrowth requires deleted in colorectal cancer-dependent MAPK activation." in: Nature, Vol. 417, Issue 6887, pp. 443-7, (2002) (PubMed).

Di Cristo, Berardi, Cancedda, Pizzorusso, Putignano, Ratto, Maffei: "Requirement of ERK activation for visual cortical plasticity." in: Science (New York, N.Y.), Vol. 292, Issue 5525, pp. 2337-40, (2001) (PubMed).

Khokhlatchev, Canagarajah, Wilsbacher, Robinson, Atkinson, Goldsmith, Cobb: "Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation." in: Cell, Vol. 93, Issue 4, pp. 605-15, (1998) (PubMed).

Li, Wysk, Gonzalez, Davis: "Genomic loci of human mitogen-activated protein kinases." in: Oncogene, Vol. 9, Issue 2, pp. 647-9, (1994) (PubMed).

Owaki, Makar, Boulton, Cobb, Geppert: "Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs." in: Biochemical and biophysical research communications, Vol. 182, Issue 3, pp. 1416-22, (1992) (PubMed).

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Abstract: MAPK1 Products

Background: Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11. 2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98 % identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins