HSP27 antibody

Catalog No. ABIN966313

This Rabbit Polyclonal antibody specifically detects HSP27 in IHC. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for HSP27 antibody (ABIN966313)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-HSP27 Antibody

Specificity: HSP27 (Ab-78) antibody detects endogenous levels of total HSP27 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-HSP27 antibody (ABIN966313)

Somara, Bitar: "Tropomyosin interacts with phosphorylated HSP27 in agonist-induced contraction of smooth muscle." in: American journal of physiology. Cell physiology, Vol. 286, Issue 6, pp. C1290-301, (2004) (PubMed).

Jordan, Brownstone, Noga: "Control of functional systems in the brainstem and spinal cord." in: Current opinion in neurobiology, Vol. 2, Issue 6, pp. 794-801, (1993) (PubMed).

Landry, Lambert, Zhou, Lavoie, Hickey, Weber, Anderson: "Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II." in: The Journal of biological chemistry, Vol. 267, Issue 2, pp. 794-803, (1992) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling